The mouse lymphoma assay
WebAbstract. In order to evaluate the utility of the mouse lymphoma assay (MLA) for detecting in vitro clastogens and spindle poisons and to compare it with the in vitro chromosomal … WebMouse lymphoma thymidine kinase locus gene mutation assay: International Workshop on Genotoxicity Test Procedures Workgroup Report
The mouse lymphoma assay
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Web14. For MLA: Because the assay was developed and characterized using the TK+/--3.7.2C subline of L5178Y cells, this specific subline must be used for the MLA. The L5178Y cell line was derived from a methylcholanthrene-induced thymic lymphoma from a DBA-2 mouse (Fischer, 1958). Clive WebMay 1, 1999 · The mouse lymphoma assay (MLA) has been widely used for many years for determining the potential for chemicals to cause the full broad spectrum of mutational damage ( Applegate et al ., 1990; Mitchell et al ., 1997 ).
WebAbstract. In order to evaluate the utility of the mouse lymphoma assay (MLA) for detecting in vitro clastogens and spindle poisons and to compare it with the in vitro chromosomal aberration test (CA), we conducted an international collaborative study of the MLA that included 45 Japanese laboratories and seven overseas laboratories under the … WebThe mouse lymphoma assay (MLA) was chosen because of a body of research indicating that many types of genetic alterations are detected. The assay detects mutations known …
WebThe mouse lymphoma assay (MLA) uses the thymidine kinase (Tk) gene of the L5178Y/Tk(+/-)-3.7.2C mouse lymphoma cell line as a reporter gene to evaluate the … WebThe Mouse Lymphoma uses cell cultures, usually mammalian, to determine gene mutations, change in chromosome structure and number, and other gene toxicities caused by medical devices, material, or their extracts. These tests are used to ensure devices that have prolonged contact with the body do not elicit genotoxicity that in turn might cause ...
WebThe "Mouse Lymphoma Assay“ enables the testing for genetic mutations caused by chemical substances and assesses the two endpoints gene mutation and chromosome damage. Abstract The permanent Mouse …
WebIntroduction to the Template for in vitro Mouse Lymphoma Thymidine Kinase Gene Mutation and Related Assays Return to Toxicology Template Introduction Table Of Contents … edina krsWebShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Storage. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. tch grooming savannahWebmicronucleus assay (note 1) and the mouse lymphoma L5178Y cell . Tk (thymidine kinase) gene mut ation assay (MLA). These three assays are currently considered equally appropriate and therefore interchangeable for measurement of chromosomal damage when used together with other genotoxicity tests in a standard battery for tchabaud/amuleWebThe mouse lymphoma assay (MLA) is used to detect gene mutations in L5178Y TK+/- -3.7.2C cells. Screening, Regulatory and Mutagenic Assessment Cultures are incubated … tcgi engineers philippinesWebThe mouse lymphoma assay was previously included in OECD guideline 476 for mammalian cell gene mutation, but was separated out into the new OECD 490 guideline in 2015. Our study director has been working on mouse lymphoma assays for > 14 years, gaining extensive experience of running this study type within industry. The mouse lymphoma … tchad kanem maoWebMay 1, 1999 · The mouse lymphoma assay (MLA) has been widely used for many years for determining the potential for chemicals to cause the full broad spectrum of mutational … tchad idriss miskineWebJan 1, 1999 · The L5178Y tk+/– mouse lymphoma assay (MLA) has been widely used as a genotoxicity test for the detection of mutagens and clastogens. The standard MLA, as well as other mammalian cell gene mutation assays, … edina kunovac zuko