Phosphotyrosine 4g10
WebApr 17, 2007 · Bound proteins were eluted with 500 mmol/L Imidazole and fractionated through 12% SDS-PAGE before their electrophoretic transfer to nitrocellulose membranes. Immobilized proteins were probed sequentially with antibodies against phosphotyrosine (4G10 antibodies; Upstate, Charlottesville, VA) followed by those against GST (Invitrogen, … WebDec 14, 2024 · b Phosphotyrosine cell content was analysed by immunoblot with anti-phosphotyrosine 4G10 mAb (pY). GAPDH immunoblot was run as loading control. In the upper panel, a representative experiment is shown. In the lower panel, the quantification of phosphotyrosine content from each condition is shown. Results are shown as …
Phosphotyrosine 4g10
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WebAnti-Phosphotyrosine Antibody, 4G10 Platinum is an antibody against Phosphotyrosine for use in WB, IF, FC, IH, IP, IC, ELISA. Research Category Signaling Research Sub Category … WebJun 15, 2012 · Phosphopeptide chip technology and antibody profile. Peptide chips containing three identical arrays of 6400 phosphopeptide spots were incubated with the indicated anti-phosphotyrosine antibodies (4G10, p-TYR-100 and pY20) and a fluorescently labelled secondary antibody.
WebThis antibody recognizes tyrosine-phosphorylated proteins from all species. Immunogen Phosphotyramine-KLH Application Anti-Phosphotyrosine Antibody, clone 4G10 detects … WebSep 8, 2015 · Here we compared two commercially available phosphotyrosine antibodies and show that P-Tyr-1000 yields 64% more phosphopeptides than 4G10 antibody, while including almost all 4G10 captured phosphopeptides. The workflow can be reproducibly performed at intermediate protein input levels of 10mg.
WebPhospho-Tyrosine (4G10) Mouse mAb recognizes a broad range of tyrosine-phosphorylated proteins and peptides. This antibody does not cross-react with proteins or peptides containing phospho-Ser or phospho-Thr residues. Species Reactivity: All Species Expected Source / Purification WebSep 19, 2016 · These data suggest that the Src superbinder may provide an alternative means to commonly used anti-phosphotyrosine antibodies 13 such as 4G10 and P-Tyr-100 for the purification of...
Web16-316 Sigma-Aldrich Anti-Phosphotyrosine Antibody, 4G10® Platinum, HRP Conjugate Download Zoom Detect Phosphotyrosine with Anti-Phosphotyrosine Antibody, 4G10 Platinum, HRP Conjugate (Mouse Monoclonal Antibody), …
Web05-1050X Sigma-Aldrich Anti-Phosphotyrosine Antibody, 4G10® Platinum Download Zoom Anti-Phosphotyrosine Antibody, 4G10 Platinum is an antibody against Phosphotyrosine for use in WB, IF, FC, IH, IP, IC, ELISA. MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. MSDS 分析证书 参考 数据表 candy and pillsWebTHE™ Phosphotyrosine Antibody (E10)plus The antibody is specific for proteins containing phosphotyrosine residues and phosphotyrosine peptides. It does not cross-react with phosphoserine, phosphothreonine or nonphosphorylated tyrosine residues. Product Details Citations Documents Overview Applications Properties Examples Background fish tank cleaning hoseWebJan 12, 2012 · Embryos were incubated with biotin-conjugated phosphotyrosine (anti-4G10, Millipore) antibody overnight at 4°C at a dilution of 1 : 500 (in block solution). Embryos were then washed (3 × 10 min) in PBDT and incubated for 2 hours at room temperature with streptavidin conjugated Cy3 or QDs 655 nm at 1 : 500 dilution in fresh block solution. fish tank cleaning spongeWebApr 12, 2024 · To measure the protein phosphotyrosine and acrosine availability, cell aliquots were fixed, permeabilized and blocked as described above (section actin polymerization). Subsequently, the samples were incubated for 1 h with specific anti-phosphotyrosine (4G10 Platinum, Sigma‒Aldrich) or anti-acrosine (ACR-2, Exbio, Praha, … fish tank cleaning syphonWebAntibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. candy and nut center fall riverWebAnti-Phosphotyrosine Antibody, clone 4G10® 05-321X Sigma-Aldrich Anti-Phosphotyrosine Antibody, clone 4G10® Download Zoom Anti-Phosphotyrosine Antibody, clone 4G10 detects tyrosine phosphorylated proteins in all species. This unique monoclonal antibody is validated for use in IC, IH, IP, WB and is backed by hundreds of More>> fish tank cleaning tools vacuumhttp://www.cnreagent.com/s/sv119268.html candy and candy ltd