Phosphinothricin resistance
WebWe have previously transformed rice (Oyrza sativa L.) with the maize ribosome-inactivating protein b-32 gene (Zmcrip3a) and the phosphinothricin resistance gene (bar). In the present study, Southern blot hybridization analysis of 56 primary fertile transformants resulted in distinct band patterns, indicating that all the transformants had been generated by …
Phosphinothricin resistance
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WebCharacterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus EMBO J. 1987 Sep;6 (9):2519-23. doi: 10.1002/j.1460-2075.1987.tb02538.x. Authors C J Thompson 1 , N R Movva , R Tizard , R Crameri , J E Davies , M Lauwereys , J Botterman Affiliation 1 Biogen S. A., 46 Route des Acacias, CH-1227 Geneva, Switzerland. WebJan 1, 2008 · The use of phosphinothricin resistance as selectable marker for genetic transformation of grapevine Authors: Rahma Jardak Centre of Biotechnology of Borj-Cedria, Tunisia Belkacem Ould Bouamama...
WebL-phosphinothricin (PPT) is a potent inhibitor of the biosynthetic enzyme glutamine synthetase (GS), which is involved in general nitrogen metabolism in plants, including the … WebOct 23, 2015 · The R295K mutation has also never been previously reported as an important residue for PPT resistance. Furthermore, the mutant gene has been transformed into …
WebL-phosphinothricin (PPT) is a potent inhibitor of the biosynthetic enzyme glutamine synthetase (GS), which is involved in general nitrogen metabolism in plants, including the assimilation of ammonia accumulated as a result of photorespiration and nitrate reduction. WebInsertion of the gene encoding phosphinothricin acetyltransferase (PAT) has resulted in cotton plants resistant to the herbicide glufosinate. However, the lower expression and commensurate reduction in PAT activity is a key factor in the low level of injury observed in the WideStrike(®) cotton and relatively high level of resistance observed in LibertyLink(®) …
WebOct 21, 2024 · The CRISPR vector for this study was used in a previously published pBAtC that is composed of the U6 promoter from Arabidopsis to express gRNA including CRISPR RNA scaffold, the 35S promoter originally from cauliflower mosaic virus (CaMV) to express Cas9 and the Basta TM (Phosphinothricin) resistance gene for the selection of the first ...
WebDMH - 11 to produce phosphinothricin-N- acetyl-transferase, the enzyme responsible for Glufosinate resistance. • Increased Productivity • Reduced import dependency. Currently, India has a deficit of edible oils by almost 55-60 per cent of the total consumption. In the financial year 2024-21, around 13.35 ... philly soft pretzel factory quakertownWebresistance of expiants to, phosphinothricin is presented. Key words: Ammonium - Carbon source - Glufosinate - Glutamine - Metabolic activity - Phosphinothricin Introduction Selectable marker genes are still a necessity in plant trans formation technology. In the presence of a selective agent, only those cells that are transformed and express the philly soft pretzel lansdale paWebApr 11, 2024 · Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20–203–01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ... philly soft pretzel moorestown njWebJan 1, 2008 · The use of phosphinothricin resistance as selectable marker for genetic transformation of grapevine R. J ARDAK -J AMOUSSI 1) , B. B OUAMAMA 1) , A. M LIKI 1) , A. G HORBEL 1) and G. M. R EUSTLE 2) philly soft pretzel factory pricesWebJul 14, 2024 · In the current study, a chloroplast transformation vector harboring the phosphinothricin resistance gene ( bar) as a selectable marker was established, and two short peptide-encoding genes ( ant 1 and ant 2) driven by the endogenous psbA and rbcL promoters were cloned in this vector. philly soft pretzel locationsWebPhosphinothricin Acetyltransferase. The phosphinothricin acetyltransferase gene is now used widely as a selective marker and also for the production of herbicide-resistant … phillysohostileWebApr 11, 2024 · ----- Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20-203-01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ... philly soft pretzel quakertown