Phis2.1
Webb24 mars 2024 · In case of 4CRM1-pHis2.1, 4CRM1mut2-pHis2.1, and 4CRM1mut5-pHis2.1, 50 mM 3-amino-1,2,4-triazole (3-AT) was sufficient to suppress background growth of yeast colonies. DNA from the TF-only prey library in pDEST_GAD424 (Mitsuda et al. 2010) was transformed into Y1HGold containing 4CRM1-pHis2.1. WebbPhis2 1 Bait Plasmids, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > phis2 1 bait plasmid. phis2 1 bait plasmid TaKaRa is a ...
Phis2.1
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Webb14 jan. 2015 · The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs ... Webb15 okt. 2024 · For the protein–DNA-binding experiment, an oligonucleotide sequence containing triple tandem copies of the W-box and mW-box was synthesised, annealed, and cloned into the pHIS2.1 vector forming W-box- and mW-box-specific reporter vectors.
WebbThe promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. However, the colonies having both constructs ... Webb1 juni 2024 · When the insertion sequence is predicted as known cis-acting element , construct the pHIS2 reporter by three tandem copies of the studied elements inserted at …
Webb1 sep. 2024 · 1-Methylcyclopropene counteracts ethylene promotion of fruit softening and roles of MiERF2/8 and MiPG in postharvest mangoes. ... and pHIS2-MiPG were cultivat ed on SD/-T r p/-His for 3 days, and . WebbPhis2 1 Bait Plasmids, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol …
Webb1 mars 2024 · C. Yeast one-hybrid assay detect the specific binding ability of VvWRKY30 to W-BOX. pGADT7-empty and pHIS2.1-W-BOX, AD- VvWRKY30 and pHIS2.1-empty were …
WebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the … oom and sheenWebbpHIS2-hsSTAM1 (1-143) Sequences pHIS2-hsSTAM1 (1-143) Sequences (2) Addgene Sequences: Partial (1) Depositing Scientist Sequences: Partial (1) Partial Sequences … iowa city little villageWebbpHIS2 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for … iowa city live newsWebb19 sep. 2024 · C-repeat binding factors/dehydration responsive element binding factors 1 (CBFs/DREB1s) are a small family of transcription factors that play important roles in plant resistance to various external stresses. However, functional characterization of tea plant (Camellia sinensis L.) CBF gene (CsCBF) was still seldom reported. Here, functional … iowa city liberty girls basketballWebb16 feb. 2024 · To explore the involvement of bZIP genes in flower senescence, one up-regulated transcript encoding a putative ocs element binding factor, namely PhOBF1 (SGN accession no. Peaxi162Scf00285g00011), was identified during petunia flower senescence through transcriptome sequencing analysis [ 36 ]. ooma not allowing internet to my computerWebbProbe 2 and Probe 4 sequences as wells mutant Probe 2M and Probe 4M sequences were cloned to pHIS2.1, which drives the expression of HIS3 . GBFs were cloned to pGADT7. Yeast cells were co-transformed with a combination of the indicated plasmids or empty vector and plated onto nonselective (−Leu/−Trp) and selective ... iowa city library loginWebb1 dec. 2024 · According to the hereditary mode of sterility genes, this vital agronomic trait can be further divided into cytoplasmic male sterility (CMS) and genic male sterility … ooma office expansion base station