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A toolbox of Cre-dependent optogenetic transgenic mice for light ...
WebInnocuous and noxious mechanical stimuli are detected by sensory neurons called low-threshold mechanoreceptors (LTMRs) and nociceptors, respectively, primarily by activating mechanically activated ion channels (10–12).In mice and humans, PIEZO2 is expressed by a subpopulation of sensory neurons and is the principal mechanically activated … WebNov 30, 2024 · 此外,角质细胞释放的tslp也能够直接刺激trpa1+的神经元以产生痒觉。上述研究为皮肤中的神经-上皮相互作用提供有力支撑,而trpv1–ai32光遗传学小鼠模型则提示纯神经信号(不依赖于上皮细胞的警报作用)也能够通过释放神经肽如cgrp以激活免疫反应,以清除 … WebMar 26, 2024 · Genetic strategies using Cre-dependent mouse strains, especially the Ai32 line expressing Channelrhodopsin (ChR2)-EYFP fusion protein, have been a popular means to drive opsin expression in a cell-type specific manner. Here we report a low level of leaky 'off-target' (Cre-independent) ChR2-EYFP expression in Ai32/Ai32 homozygous mice … standard fax cover sheet free